Generating controlled, dynamic chemical landscapes to study microbial behavior

Abstract

We demonstrate a method for the generation of controlled, dynamic chemical pulses―where localized chemoattractant becomes suddenly available at the microscale―to create micro-environments for microbial chemotaxis experiments. To create chemical pulses, we developed a system to introduce amino acid sources near-instantaneously by photolysis of caged amino acids within a polydimethylsiloxane (PDMS) microfluidic chamber containing a bacterial suspension. We applied this method to the chemotactic bacterium, Vibrio ordalii, which can actively climb these dynamic chemical gradients while being tracked by video microscopy. Amino acids, rendered biologically inert ('caged') by chemical modification ..